High-Throughput Screening (HTS) for Drug Discovery

OTRADI conducts high-throughput drug screening of novel compounds against a variety of targets. It offers the following services:

  • Aids in lead compound optimization
  • Analyzes compound potency using dose-response curves
  • Compares activity of compounds to validated control drug activity
  • Identifies lead compounds with drug-like activities
  • Prepares cytotoxicity profiles for compounds
  • Prioritizes hits for further characterization
  • Rapidly identifies biologically active compounds
  • Tests compounds for novel biological effects and unique bioactivity

Assay Development to Automate and Optimize Drug Discovery

OTRADI develops novel assays to automate drug discovery. It has optimized protocols, which are scaled and validated for high-throughput screening of the following:

  • Anti-bacterial assays
  • Anti-infectious disease assays
  • Anti-malarial assays
  • Anti-viral assays
  • Assays for important therapeutic enzymatic 'targets'
  • Cell-based assays
  • Cytotoxicity assays
  • Genomic assays
  • Immunoassays
  • Kinase-profiling assays
  • Novel assays using fluorescence, luminescence and spectrophotometric read-outs
  • Proteomic assays

High-Content Analysis (HCA) for Cell Analysis, Compound Screening and Compound Profiling

OTRADI performs automated fluorescence microscopy for cellular imaging and analysis. OTRADI has the capability to:

  • Scale-up routine laboratory cell-staining assays
  • Optimize protocols and select appropriate probes
  • Acquire high-throughput images
  • Develop and modify algorithms for cellular image analysis
  • Design and validate image-based cell screening assays for attributes including cell counting, cell health, biomarker intensities, activity probe intensities, organelle morphometric parameters, biomarker translocation, biomarker correlation/co-localization and texture analysis
  • Profile compound activity and toxicity